James G. Wigmore worked as a forensic alcohol toxicologist at one of the foremost forensic laboratory in North America -- the Centre of Forensic Sciences in Toronto, Ontario -- for over twenty-nine years. He has testified in over 700 criminal cases throughout Canada, in personal injury civil cases, coroner's inquests, Alcohol and Gaming Commission of Ontario hearings, and a military court martial in Germany. He has published numerous scientific studies in this area, which have been cited by the Supreme Court of Canada (R v St. Onge-Lamoreux, 2012). In 2005 he was awarded the prestigious Derome Award from the Canadian Society of Forensic Science in recognition of outstanding contributions in the field of forensic science. Between 2004 and 2012, he taught on the Robert F. Borkenstein Course at Indiana University in Bloomington.

He has presented many seminars and alcohol workshops to numerous medicolegal organizations, including the Ontario Crown Attorneys, the California DUI Lawyers Association, the Southwestern Association of Forensic Scientists (Texas), the Osgoode Hall Law School (York University), and at various judges' meetings.