Forewords.- Introduction to Immunoassays.- Antibodies.- ELISA/EIA/FIA.- Sample Preparation for Immunoassays.- Lateral Flow Immunoassays.- Immuno-PCR: Application-oriented highly sensitive protein analytics with antibody-DNA conjugates.- Evolution of PCR - from classical PCR to digital PCR.
- Detection of Proteins on Membranes: Western Blot and Dot Blot Methods.- Acceleration of Western Blotting.- Lab-on-a-Chip - Miniaturization of Assays for On-Site Diagnostic Applications Using Microfluidics.- Reliable protein quantification by surface plasmon resonance spectroscopy.- Troubleshooting and Assistance in the Development, Validation and Implementation of ELISAs.- Detection Methods for Protein Interaction.- Immunohistochemistry for Research and Diagnostic Pathology.- Fluorescence Microscopy in Immunoassay Application - An Introduction.
- In Vivo Immunostaining and Intravital Two-Photon Microscopy.- Quantitative digital holographic phase contrast microscopy - biophysical characterization of immunological processes, cells and tissues.- Circulating Tumor Cells - Isolation and Molecular Characterization.- Automated isolation of circulating tumor cells for liquid biopsy.- 3D Cell and Organoid Culture.- Tissue Engineering and Regenerative Medicine.- The CRISPR/Cas Technology: Classic and Future Applications in Molecular Biology and Medicine.- Fluorescent Dyes.
- Stabilization of Assay Components.- Interference Effects in Immunoassays.- Troubleshooting and Assistance in the Development, Validation and Implementation of ELISAs.- Evaluation and Validation.- Food and Feed Analysis.- Automation of Immunoassays.- Challenges for a Next Generation Biobanking - Cryopreservation for the advancement of personalized medicine.- The new EU Regulation 2017/746 on in vitro diagnostic medical devices.
- ISO 13485 - the mandatory quality system for IVD manufacturers.- Clinical Trials for In-vitro Diagnostics.- Protection for Diagnostic Methods and its Limits under the European Patent Convention and the German Patent Law.- Learning Laboratory Systems: How can artificial intelligence support laboratory work?.- Impedance-based Analysis of Functional Immunoassays using Cultured Cells.- Measurement of Single Molecules and Cells with Atomic Force Microscopy.- Immunoassays for Extracellular Vesicles-based Characterization.- Immunoassay Fabrication and Device Functionalization by Scanning Probe Lithography.
- The digital lab of the future, laboratory information systems, analytics and AI in LifeScience R&D.- Regulation of Medical Devices in the USA with a Focus on IVDs.- Optical Coherence Tomography for Non-Invasive Imaging of Tissue and Organoids.- sFIDA: Quantitative Single Particle Analysis.- The Blood-Brain Barrier.- The Biomedical Lab, Ethics, and Obtaining Ethics Approval for Research.- A label-free future: digid´s BioMEMS platform enables diagnostics beyond the laboratory.- Ethical Boundaries of Research Freedom.
- New Approaches in Personalized Tumor Therapy Research: Organoids, Organoid-on-Chip, and Tumor Explants as Models.