Isolation of Epidermal Keratinocytes from Human Skin: The Scratch-Wound Assay for Assessment of Epidermal Keratinocyte Migration.- Isolation of Different Dermal Fibroblast Populations from the Skin and the Hair Follicle.- Isolation of Epidermal and Hair Follicle Melanocytes.- Validated Step-By-Step Protocols for Human Keratinocyte Isolation and the Construction of Organotypic 3D Epidermal Models.- Human Epidermal Equivalents on Non-Woven Scaffolds: A Model of Tissue Formation in an Artificial Environment.- Purification of Extracellular Vesicles Secreted by Skin Fibroblasts and Keratinocytes.- Genetic Engineering of Human Keratinocytes using CRISPR/Cas9 Ribonucleoprotein Complexes or Modified Cas9-Encoding mRNAs.- Imaging Approaches to DNA Damage Induction and Quantification.
- Identification of Long Non-Coding RNA by In Situ Hybridization Approaches.- Detection of MicroRNAs in Skin by In Situ Hybridization.- 3D-FISH Analysis of the Spatial Genome Organization in Mammalian Tissue in Situ.- Methods to Investigate the Epidermal Permeability Barrier and the Light Irradiation Barrier In Vitro and Ex Vivo.- A Protocol to Prepare and Stain Dorsal Skin Whole Mounts from Mice.- Isolation of Mouse Hair Matrix Keratinocytes for Multi-Omics Analysis.- Methods to Study Human Hair Follicle Growth Ex Vivo: Human Microdissected Hair Follicle and Human Full Thickness Skin Organ Culture.- Generation of Hair Follicle Germs In Vitro Using Human Postnatal Skin Cells.
- In Situ Laser Capture Microdissection for Detection of Components of the Hair Follicle and Scalp Microbiome.- Viable vs. Non-Viable Microbiota Evaluation of the Hair Follicle and Scalp Microbiome.- Non-Surgical Induction of Alopecia Areata in C3H/HeJ Mice via Adoptive Transfer of Cultured Lymphoid Cells.- Collapse and Restoration of Hair Follicle Immune Privilege Ex Vivo: A Model for Alopecia Areata.- Experimentally Induced Epithelial-Mesenchymal Transition of Human Hair Follicle Stem Cells as a Model of Scarring Alopecia Ex Vivo.- Method for Human Sweat Gland Isolation from the Scalp and Axilla by Means of the Micro-Punch FUE Technique and Vital Dyes.- Assessment of Acute Wound Healing using Excisional Mouse Tail Skin Wound Models.
- Skin and Wound Infection Models to Test Host-Pathogen Interactions and Evaluate Topical Antimicrobial Treatments.- Human Wound Healing Ex Vivo Model with Focus on Molecular Markers.- The Evaluation of Fibrotic Alterations in Regenerated Murine and Human Xenografted Skin.- Method to Study Skin Cancer: Two-Stage Chemically Induced Carcinogenesis in Mouse Skin.- Ultraviolet Irradiation of Skin Cells.- Method for Investigation of Photobiological Effects of Light on Human Skin Cells In Vitro Using Visible and Near-Infrared Light.