This third edition provides protocols on fluorescence in situ hybridization (FISH) and comparative genomic hybridization approaches. Chapters provide new and updated methods on probe, cell types, tissues, organisms, plants, microorganisms, multicolor FISH procedures, chromosomes, breakpoints, cryptic aberrations, nuclear architecture, epigenetic changes, and comparative genomic hybridization studies, immunostaining, single cell electrophoresis, microdissection, sequencing, and CRISP/Cas9 technology. Authoritative and cutting-edge, Fluorescence In Situ Hybridization (FISH): Application Guide, Thid Edition aims to be useful to researchers just starting to study FISHers and for FISH experts.