Preface. Table of Contents. Contributing Authors. 1. Important Considerations for Elispot Validation Sylvia Janetzki 2. Current Trends in Validating Antibody Specificities for Elispot by Western Blotting Biji T. Kurien and R. Hal Scofield 3.
An Overview of Peptides and Peptide Pools for Antigen-Specific Stimulation in T-Cell Assays Karsten Schnatbaum, Pavlo Holenya, Sebastian Pfeil, Michael Drosch, Maren Eckey, Ulf Reimer, Holger Wenschuh, and Florian Kern 4. The Applications of ELISpot in the Identification and Treatment of Various Forms of Tuberculosis and in the Cancer Immunotherapies Hemant K. Mishra 5. Artificial Intelligence-Based Counting Algorithm Enables Accurate and Detailed Analysis of the Broad Spectrum of Spot Morphologies Observed in Antigen-Specific B Cell ELISPOT and FluoroSpot Assays. Alexey Y. Karulin, Melinda Katona, Zoltán Megyesi, Greg A. Kirchenbaum, and Paul V. Lehmann 6.
Comparing Flow Cytometry and ELISpot for Detection of IL-10, IL-6, and TNF Alpha on Human PBMCs Kristina Boss, Jodi Hagen, Megan Constans, Christine Goetz, and Alexander E. Kalyuzhny 7. Reagent Tracker(tm) Platform Verifies and Provides Audit Trails for the Error-Free Implementation of T Cell ImmunoSpot® Assays Alexander A. Lehmann, Diana R. Roen, Zoltan Megyesi, and Paul V. Lehmann 8. Detection of SARS-CoV-2-Specific Cells Utilizing Whole Proteins and/or Peptides in Human PBMCs using IFN- ELISpot Assay Madeleine M. Rasche, Ella C.
Kaufmann, Tamar Ratishvili, Ilya M. Swanson, Inna G. Ovsyannikova, and Richard B. Kennedy 9. Interferon-γ/IL-2 ELISpot and mRNA Responses to the SARS-CoV2, Feline Coronavirus Serotypes 1 (FCoV1), and FCoV2 Receptor Binding Domains by the T Cells from COVID-19-VaccinatedHumans and FCoV1-Infected Cats Sabarinath Nair, Bikash Sahay, Ananta P. Arukha, Lekshmi K. Edison, Chiquitha D. Crews, John G.
Morris Jr., Subhashinie Kariyawasam, and Janet K. Yamamoto 10. Frequencies of SARS-CoV-2 Spike Protein-Specific Memory B Cells in Human PBMCs, Quantified by ELISPOT Assay Ilya M. Swanson, Iana H. Haralambieva, Madeleine M. Rasche, Inna G. Ovsyannikova, and Richard B.
Kennedy 11. Monitoring Memory B Cells by Next Generation ImmunoSpot® Provides Insights into Humoral Immunity that Measurements of Circulating Antibodies Do Not Reveal Paul V. Lehmann, Zhigang Liu, Noémi Becza, Alexis V. Valente, Junbo Wang, and Greg A. Kirchenbaum 12. Tracking Circulating HLA-Specific IgG-Producing Memory B Cells with the B Cell ImmunoSpot Assay Delphine Kervella, Sebastiaan Heidt, Robert Fairchild, Stephen Todryk, and Oriol Bestard 13. Assessing the Affinity Spectrum of the Antigen-Specific B Cell Repertoire via ImmunoSpot® Noémi Becza, Zhigang Liu, Jack Chepke, Xing-Huang Gao, Paul V. Lehmann, and Greg A.
Kirchenbaum 14. Using PBMCs in a Multiplex FluoroSpot Assay for Detection of Innate Immune Response Modulating Impurities (IIRMIs) Bartek Makower and Niklas Ahlborg 15. Four-Color ImmunoSpot® Assays Requiring Only 1-3 mL of Blood Permit Precise Frequency Measurements of Antigen-Specific B Cells Secreting Immunoglobulins of All Four Classes and Subclasses Lingling Yao, Noémi Becza, Andrea Maul-Pavicic, Jack Chepke, Greg A. Kirchenbaum, and Paul V. Lehmann 16. High-Plex Elispot: FOLISPOT Based on Fluorescence Detection and DNA Complementary Pairing Mi Liu 17. Triple-Color FluoroSpot Analysis of Polyfunctional Antigen-Specific T Cells by Quantification of Spot-Forming Units and Relative Spot Volumes Niklas Ahlborg, Christian Smedman, and Bartek Makower 18. Performance and Stability of New Class of Fetal Bovine Sera (FBS) and Its Lyophilized Form in ELISpot and FluoroSpot Assays: Applications for Monitoring the Immune Response in Vaccine, and Cell and Gene Immunotherapy in Clinical Trials Zincous Hosseini, Christopher J.
Groves, Penny Anders, Kristen Cave, Madelyn Krunkosky, Brandi Chappell, Sofie Pattyn, Devin Davis, Sylvia Janetzki, and Elizabeth Reap Subject Index List.