Volume 1 Editorial Board xi Contributors xiii Preface xxiii Author and Editor Conflicts of Interest xxv Section A Molecular Methods 1 Volume Editor: John L. Schmitz Section Editor: Eric T. Weimer 1 Introduction 3 Eric T. Weimer 2 Molecular Methods for Genetic Diagnosis of Inborn Errors of Immunity 5 Sarah L. Anzick and Amy P. Hsu 3 Immunoglobulin and T- Cell Receptor Gene Assessment 23 Paul A. Wadsworth, Chandler C. Ho, and Bing M.
Zhang Section B Immunoglobulin Methods 39 Volume Editor: Barbara Detrick Section Editor: David F. Keren 4 Introduction 41 David F. Keren 5 Clinical Indications and Applications of Serum and Urine Protein Electrophoresis 43 David M. Manthei and David F. Keren 6 Immunochemical Characterization of Immunoglobulins in Serum, Urine, and Cerebrospinal Fluid 62 Caitlin O. Schein and William G. Finn 7 Cryoglobulins and Cryofibrinogenemia 79 Peter D. Gorevic, Dennis Galanakis, and Jeffrey S.
Warren 8 Strategy for Detecting and Following Monoclonal Gammopathies 90 David F. Keren and Lee Schroeder Section C Complement 107 Volume Editor: Barbara Detrick Section Editor: Ashley Frazer-Abel 9 Introduction 109 Ashley Frazer- Abel 10 Methods of Complement Analysis 111 Diana Pauly and Michael Kirschfink 11 Assessing Complement Dysregulation in the Thrombotic Microangiopathies and Ultrarare Complement- Mediated Kidney Disease 119 Amanda K. Slagle, Jill J. Hauer, and Richard J. H. Smith 12 Current and Future of Complement Therapeutics and their Impact on the Clinical Immunology Laboratory 129 Bradley Dixon, Shrey Purohit, and Ashley Frazer- Abel Section D Flow Cytometry 139 Volume Editor: Maurice R. G. O''gorman Section Editor: Paul K.
Wallace 13 Introduction: Clinical Flow Cytometry Applications and Quality Control 141 Paul K. Wallace 14 Polychromatic Flow Cytometry 144 Ankit Saxena, Angelique Biancotto, Pradeep Dagur, And John Philip Mccoy, Jr 15 High- Sensitivity Detection of Paroxysmal Nocturnal Hemoglobinuria by Multiparameter Flow Cytometry 166 Ahmad Al- Attar, Stephen J. Richards, Iuri Marinov, Andrea Illingworth, And D. Robert Sutherland 16 Standardized Flow Cytometry Assays for Enumerating CD34 + Hematopoietic Stem Cells 189 Ahmad Al- Attar and D. Robert Sutherland 17 The Detection of Fetal Red Cells in Fetomaternal Hemorrhage by Flow Cytometry 213 Bruce Greig 18 Functional Flow Cytometry- Based Assays of Myeloid and Lymphoid Functions for the Diagnostic Screening of Inborn Errors of Immunity 221 Caleb Cornaby and Maurice R. G. O''gorman 19 Quality Assurance and Validation in Clinical Flow Cytometry 231 Teri Oldaker and Antony Bakke 20 Future Cytometric Technologies and Applications 249 Holden T. Maecker Section E Functional Cellular Assays 253 Volume Editor: Maurice R.
G. O''gorman Section Editors: Miranda B. Carper And Thomas N. Denny 21 Introduction 255 Miranda B. Carper and Thomas N. Denny 22 Cryopreservation of Blood Mononuclear Cells 257 Cassandra Porth, Ambrosia Garcia, Raul Louzao, Krystle Garth, Emilie Jalbert, and Adriana Weinberg 23 Functional Assays for B Cells and Antibodies 266 David C. Lafon and Moon H. Nahm 24 Methods for Detection of Antigen- Specific T Cells by Enzyme- Linked Immunospot Assay 277 Mark Berrong, Danielle Nettere, and Guido Ferrari 25 Regulatory T Cell (Treg) Characterization: Phenotype, Function, and Clinical Importance of Human Treg 284 Alma- Martina Cepika and Rosa Bacchetta 26 Measurement of Natural Killer Cell Phenotype and Activity in Humans 292 Heinrich Schlums, Tessa M.
Campbell, and Yenan T. Bryceson 27 Functional Assays for the Diagnosis of Chronic Granulomatous Disease 304 Debra Long Priel and Douglas B. Kuhns 28 Interferon Gamma Release Assays for Tuberculosis Diagnosis 318 Sofia Zavala and Jason E. Stout 29 Methods for Evaluating Antigen- Specific Cytotoxic T Lymphocyte Frequency and Activity 324 Danielle Nettere and Guido Ferrari Section F Cytokines 333 Volume Editor: Barbara Detrick Section Editors: John J. Hooks And Timothy B. Niewold 30 Introduction 335 John J. Hooks and Timothy B. Niewold 31 Overview of the Cytokine Assay Multiverse: Systems and Applications 337 Julio C.
Valencia, Ngoc Thuy T. Nguyen, And Howard A. Young 32 Monitoring Interferons in Autoimmune Diseases and Interferonopathies 344 Andrra Nimoni and Timothy B. Niewold 33 Detection of Anti-Cytokine Autoantibodies and Clinical Applications 352 Serena J. Lee, Eszter Toth, Lindsey B. Rosen, Sarah K. Browne, and Steven M. Holland Section G Laboratory Management 363 Volume Editor: John L.
Schmitz Section Editor: Thomas S. Alexander 34 Licensure and Accreditation 365 Thomas S. Alexander 35 Validation and Quality Control: General Principles and Application to the Clinical Immunology Laboratory 369 Terri Lebo, Patricia A. Merkel, and Vijaya Knight 36 Quality Assurance and Quality Management of the Medical Laboratory 384 Paul R. Johnson Section H Infectious Diseases Caused By Bacteria, Mycoplasmas, Chlamydiae, and Rickettsiae 393 Volume Editor: John L. Schmitz Section Editor: Christine M. Litwin 37 Introduction 395 Christine M. Litwin 38 Diagnostic Methods for Group A Streptococcal Infections 396 Christine M.
Litwin, Sheldon E. Litwin, And Harry R. Hill 39 Laboratory Diagnosis of Syphilis 404 Tyson Chiaro and John L. Schmitz 40 Lyme Disease, Relapsing Fever, and Leptospirosis 412 Guiqing Wang and Maria E. Aguero- Rosenfeld 41 Immunological Tests in Tuberculosis 424 Christine M. Litwin 42 Mycoplasma: Serological Methods 433 Ken B. Waites, Mary B. Brown, and Jerry W.
Simecka 43 Chlamydia Infections 438 Rosemary C. She and Valentina Zanfagnin 44 The Rickettsiaceae, Anaplasmataceae, and Coxiellaceae 447 Lucas S. Blanton and David H. Walker 45 The Bartonellaceae, Brucellaceae, and Francisellaceae 458 Christine M. Litwin, Burt Anderson, Renee Tsolis, and Amy Rasley Section I Mycotic and Parasitic Diseases 467 Volume Editor: John L. Schmitz Section Editor: Elitza S. Theel 46 Introduction 469 Elitza S. Theel 47 Immunological and Molecular Approaches for the Diagnosis of Parasitic Infections 470 Marc R.
Couturier and Elitza S. Theel 48 Serological Diagnosis of Fungal Infections 489 Mark D. Lindsley Section J Viral Diseases 521 Volume Editor: John L. Schmitz Section Editor: Jennifer Dien Bard 49 Introduction 523 Jennifer Dien Bard 50 Herpes Simplex Virus and Varicella- Zoster Virus 524 Susan Realegeno 51 Epstein- Barr Virus and Cytomegalovirus 535 Henry H. Balfour, Jr., Kristin A. Hogquist, And Priya S. Verghese 52 Human Herpesviruses 6A, 6B, 7, and 8 550 Shangxin (shaun) Yang 53 Parvovirus B19 563 Rebecca Yee and Jose A.
Lucar 54 Severe Acute Respiratory Syndrome Coronavirus- 2 572 Patricia Slev 55 Enteroviruses 583 Bijal A. Parikh and Allison R. Eberly 56 Measles, Mumps, and Rubella Viruses 592 Ryan F. Relich and Elitza S. Theel 57 Viral Hepatitis 600 Kileen L. Shier 58 Arboviruses 616 Mimi R. Precit and Rachael M. Liesman 59 Hantavirus Infections 634 William Marciel De Souza and Luiz Tadeu Moraes Figueiredo 60 Rabies Virus 640 D.
Craig Hooper 61 Human T- Cell Lymphotropic Virus Types 1 and 2 649 Laura Gillim and Ajay Grover 62 Human Immunodeficiency Virus 658 S. Michele Owen 63 Bandavirus 668 Amanda E. Calvert and Holly R. Hughes Volume 2 Section K Errors Of Immunity 679 Volume Editor: Maurice R. G. O''gorman Section Editor: Roshini S. Abraham 64 Introduction to Inborn Errors of Immunity 681 Roshini S. Abraham 65 Newborn Screening for Severe Combined Immunodeficiency 683 Lisa Kobrynski and Mirjam Van Der Burg 66 Combined Immunodeficiencies 692 Tamar S.
Rubin, Kelli W. Williams, and Monica G. Lawrence 67 Predominantly Antibody Deficiencies 717 Sara Barmettler, Daniel Digiacomo, and Jocelyn R. Farmer 68 Primary Immune Regulatory Disorders 732 Shanmuganathan Chandrakasan, Gulbu Uzel, Carrie L. Lucas, And Andrew L. Snow 69 Primary Innate Immune Defects (Including Phagocytic Disorders, TLR Defects, and NK Cell Deficiencies) 752 Jenna R. E. Bergerson, Alexandra F.
Freeman, Emily M. Mace, And Keith A. Sacco 70 Systemic Autoinflammatory Diseases 768 Xiao Peng, Oskar Schnappauf, Adriana A. De Jesus, and Ivona Aksentijevich 71 Complement Defects 796 Ann M. Moyer, Meera Sridharan, and Maria Alice.