Overview of chikungunya virus epidemiology, biology, and pathogenesis.- Naked-eye visual loop-mediated isothermal amplification method for the rapid detection of chikungunya virus.- RT-RPA assisted CRISPR/Cas12a based Isothermal Detection of Chikungunya virus.- Quantification of Chikungunya Virus RNA by Real-Time RT-PCR Assay.- Determination of Chikungunya Virus Infectious Titer Using Plaque Assays.- Automated Counting of Chikungunya Virus&Formed Plaques.- Production of Viral Particles from a Chikungunya Virus Infectious Clone.- Molecular Cloning and Reverse Genetics.
- A Restriction-Free Cloning Approach for Molecular Engineering of Plasmids.- Viral Protein Expression and Purification.- PAGE-based measurements of nsP2 protease activity.- Assessing Chikungunya Virus Protease Activity and Pharmacological Inhibition Using the acc-CHIK15-dnp Fluorogenic Peptide Substrate .- Epitope tagging and coimmunoprecipitation to identify viral protein interactors.- Plaque reduction neutralization test against chikungunya virus using liver homogenate.- Screening of Antiviral Agents against CHIKV using reporter virus.- In silico drug design of chikungunya antivirals: using consensus methods for virtual screening.
- Surface Plasmon Resonance-Based Analysis of Ligand Binding Kinetics in Chikungunya Virus Drug Discovery.- Analysis of cell populations from arbovirus infected tissues by flow cytometry.- Chikungunya virus infection in paraffin-embedded tissue: analysis of histological alterations and viral detection by immunohistochemistry.- Collection and processing of adult mosquitoes (Diptera: Culicidae) for molecular studies of arbovirus infection.- Assessing the Vector Competence for Chikungunya Virus in Mosquitoes.- Global Genomic Surveillance.- Nanopore Sequencing for Chikungunya Virus: Principles and Application.- Workflow for Long-Read Amplicon Sequencing of Chikungunya Virus Using Oxford Nanopore Technology .
- Optimized Amplicon Strategy for Long-Read Sequencing of the Chikungunya Virus Genome.- Protocol for detecting and sequencing chikungunya virus from field-collected mosquitoes.