Selecting Novel Retino-Tropic AAV Capsids by Directed Evolution.- Evaluation of CRISPR/CasRx-Mediated VEGF mRNA Knockdown in Mouse Retina.- Optimized Retinal Cell Dissociation for Downstream Analysis of Cone Photoreceptors.- Development of In Vitro Potency Assays for Gene Therapy Products Targeting cGMP-Related Retinopathies.- Quantification of Drusen-Like Deposits in Human Pluripotent Stem Cell-Derived Retinal Pigment Epithelium Cells Using Confocal Microscopy.- Ex Vivo Culture and Formalin-Fixed Paraffin Embedding of Murine Retinal Explants.- Culture of Human Retinal Explants as an Ex Vivo Model for Retinal Gene Therapy.- Electrophysiological Characterization of Photoreceptor-Like Cells in Retinal Organoids Using Whole-Cell Patch Clamp Method.

- Generation and Characterization of Patient-Derived Human Pluripotent Stem Cell Lines for Retinal Gene Therapy.- Transduction of AAV Vectors in iPSC-Derived Retinal Organoids.- Intravitreal Injections in Mouse Models of Eye Disease.- Behavioral Assessment of Vision in Dogs Using a Four-Alternative Choice-Based Testing Device.- Behavioral Vision Testing Using an Automated Observational Tracking System (VisioBox) to Obtain a Measure of Visual Acuity in Larval Zebrafish.- Design and Validation of Mirtron-Mediated RNA Interference for Gene Therapy.- Applications of Fundus-Tracked Perimetry in the Assessment of Visual Sensitivity.- Assessing Visual Function in Retinal Gene Therapy.