The Immunology of Transfusion Medicine: Past, Present, and Future.- Characterization of Platelet and Red Blood Cell Extracellular Vesicles by Spectral Flow Cytometry.- Mouse Models of Platelet Alloimmunization and Refractoriness.- Generation and Use of an Alloantigen-Specific Preclinical Humanized Mouse Model of Fetal and Neonatal Alloimmune Thrombocytopenia.- Mouse Model of Hemolytic Disease of the Fetus and Newborn.- Anti-PF4/Heparin Antibody Production in a Mouse Immunization Model.- Microscopy-Based Methods for Measuring Platelet and Red Blood Cell Phagocytosis.- Miniaturized In Vitro Transfusion Model.
- Mouse Models of Immune Thrombocytopenia.- Methods to Examine Complement Activation by HIT Immune Complexes.- Cloning of Antibodies from Patients with Heparin-Induced Thrombocytopenia.- A Detailed Protocol to Induce and Treat Passive Immune Thrombocytopenia in Mice.- Platelet Transfusion in Murine Models of Neonatal Thrombocytopenia.- Quantification of Human Platelet Clearance In Vitro and In Xenotransfused Animals.- Tracking and Testing Transfused Versus Endogenous Platelets with Biotin or Human Leukocyte Antigen.- Analysis of Biotinylated Red Cell Blood Cells Following Transfusion.
- Examination of Antigen-Specific B Cell Responses to RBC Transfusion.- Determining the Binding Affinity in Monoclonal Antibody-Alloantigen Interactions.- Modeling the Application of Chimeric Antigen Receptor T Cell Therapy to Eliminate Alloantibodies in Mice.- Examination of Class I HLA-Fc Fusion Proteins in Targeting Cognate Antibody-Producing Cells.- Isolation and Characterization of Mesenchymal Stem Cells from Human Umbilical Cord Tissue.- Prolonging the Lifespan of Neutrophils for Granulocyte Transfusion in Neutropenic Mice with Bacterial Pneumonia.- Mouse Models of Transfusion-Related Acute Lung Injury (TRALI).- Methodological Approaches to Study Transfusion-Related Acute Lung Injury (TRALI): From Complement Activation to In Vivo Mouse Model.
- High-Throughput Metabolomics in Transfusion Medicine.- Assessing the Impact of Intravascular Hemolysis on Erythropoiesis in Sickle Cell Disease.- Murine Models of Transfusion-Induced Red Blood Cell Alloimmunization.- Establishing, Maintaining, and Cryopreserving In Vitro Cultures of the Asexual Stages of Babesia divergens.- Building the Toolkits to Examine the Expression and Associated Regulation of RBC Alloantigens.- Use of Microbial Microarrays to Define Antibody Specificity.- Production of Induced Pluripotent Stem Cell-Derived Red Blood Cells.- Red Blood Cell Storage and Post-Transfusion Recovery.
- The Use of Bioengineered Induced Pluripotent Stem Cell-Derived Human Megakaryocytes to Diagnose and Characterize Platelet Allo-, Auto- and Iso-Immune Disorders.- Defining the Specificity of Blood-Group Binding Lectins through Microarray Analysis.- Characterization of Antigen Specific CD4+ T Cell Responses to the Transfusion of Red Blood Cells.- Defining the Role of Dendritic Cells in Red Blood Cell Alloimmunization.- Examination of the Immunological Role of Toll-Like Receptor 9 on Red Blood Cells.- Examining Inflammatory Effects of Reticulocyte Erythrophagocytosis in Human Macrophage Cultures.- Anemia and Transfusion in Preclinical Models of Neonatology.- Expression and Characterization of Blood Group Binding Lectins.
- Acute Incompatible Red Blood Cell Transfusion in Mice.- In Vitro Phagocytosis Assays.- Analysis of Galectin Binding to Blood Group Expressing Bacteria.- Western Blot Detection of Blood Group A and B Antigens.- Flow Cytometric Examination of Galectin Binding to Red Blood Cells.- Analysis of Erythrocyte Membrane Alloantigens.- Extraction and Quantification of Skin Immune Cells and Skin Lipids.- Defining the Role of Marginal Zone B Cells in RBC Alloimmunization.
- A Flow Cytometric Methods of Determining CD71+ Erythroid Cells in Neonatal Whole Blood.- Defining the Role of Marginal Zone B Cells in FVIII Inhibitor Development.