Section I: Methods 1. Overview of Technical Aspects and Chemistries of Next-Generation Sequencing 2. Targeted Hybrid Capture Methods 3. Amplification-Based Methods 4. Emerging DNA Sequencing Technologies 5. Transcriptomics, RNA-Sequencing and Methylome Analysis Section II: Bioinformatics 6. Base Calling, Read Mapping, and Coverage Analysis 7. Single Nucleotide Variant Detection Using Next Generation Sequencing 8.

Insertions and Deletions (Indels) 9. Translocation Detection Using Next-Generation Sequencing 10. Copy Number Variant Detection Using Next-Generation Sequencing 11. Reference Databases for Disease Associations 12. Reporting of Clinical Genomics Test Results 13. Reporting Software 14. Constitutional Diseases: Target enrichment strategies for Targeted gene sequencing panels 15. Targeted Hybrid Capture for Inherited Disease Panels 16.

Constitutional Disorders: Whole Exome and Whole Genome Sequencing 17. Somatic Diseases (Cancer): Amplification-Based Next-Generation Sequencing 18. Targeted Hybrid-Capture for Somatic Mutation Detection in the Clinic 19. Somatic Diseases (Cancer): Whole Exome and Whole Genome Sequencing 20. Assay Validation 21. Regulatory Considerations Related to Clinical Next Generation Sequencing 22. Genomic Reference Materials for Clinical Applications 23. Ethical Challenges in Clinical Genomics 24.

Legal Issues 25. Billing and Reimbursement Section III: Clinical Informatics and IT Infrastructure 26. Cloud Computing for Clinical NGS Testing Section III: Interpretation 27. Bioinformatics of Long Read Sequencing Section IV: Regulation, Reimbursement, and Legal Issues 28. Business aspect of Precision Genomic Medicine 29. Driving precision medicine through genomics-EHR integration.