Expression and Purification of Recombinant Macrophage Migration Inhibitory Factor (MIF).- Generation of MIF Peptides via Solid Phase Peptide Synthesis.- E xpression and Purification of Recombinant D-Dopachrome Tautomerase (D-DT).- S tructural Determination and Ligand Interaction of Macrophage Inhibitory Factor: A Standardized X-Ray Crystallography Approach.- Generation of High-Quality D-Dopachrome Tautomerase Crystals for Structural Studies.- Fragment Screening of MIF by Surface Plasmon Resonance.- D etection of Noncovalent Protein-Ligand Complexes of D-DT Variants by MALDI-TOF Mass Spectrometry.- Analysis of D-Dopachrome Tautomerase Conformational Flexibility Through Molecular Dynamics Solutions.
- MIF NMR Chemical Shift Perturbation Mapping.- Assessing the Tautomerase Activity of MIF Using L-Dopachrome.- Using 4-HPP and PP as Substrates to Assess Purified MIF and D-DT Tautomerase Activity and Inhibition.- Protocols for Assessing the Nuclease Activity of MIF.- Detection of Oxidized Macrophage Migration Inhibitory Factor (oxMIF) in Biological Samples Using Enzyme-Linked Immunosorbent Assay (ELISA).- Detection of Oxidized Macrophage Migration Inhibitory Factor (oxMIF) in Tissue Samples Using Immunohistochemistry (IHC).- E xploring the Chemotactic Effects of MIF on Neutrophils and T Cells and Methods for Selective Receptor Targeting.- Polarization of Bone Marrow-Derived Macrophages from Wild-Type and High Human MIF-Expressing CATT7 Polymorphic Mice.
- Using Luciferase Reporter Cells to Assess the Effects of MIF Inhibitors on NF- k B Activation.- Monitoring Inflammasome Activity through ASC Speck Formation in THP-1 ASC-GFP Cells.- Small Interfering RNA (siRNA)-Mediated Knockdown of Macrophage Migration Inhibitory Factor Transcript in Adherent and Suspension Cultures of Human Cell Lines.- Stable Transfection ofCD74Gene Variants into A549 Lung Adenocarcinoma Cell Line.- Methods to Determine the Role of MIF in Cilia Biogenesis.- Modelling Fungal and Amoebic Infections: Co-Culture Approaches to Study MIF&s Role in Infection Induced Inflammatory Responses.- Immunofluorescent Quantification of MIF in Larval Zebrafish Tailfin Transfection.