Measuring GPCR-Induced Intracellular Calcium Signaling Using a Quantitative High-Throughput Assay.- Quantifying Gq Signaling Using the IP1 Homogenous Time Resolved Fluorescence (HTRF) Assay.- Measuring IP3 Generation in Real-Time Using a NanoBiT Luminescence Biosensor.- Monitoring Calcium-Sensing Receptor (CaSR) Induced Intracellular Calcium Flux Using an Indo-1 Flow Cytometry Assay.- Bioluminescence Resonance Energy Transfer (BRET) Assay to Measure Gq Recruitment to the Ghrelin Receptor.- Analysis of Calcium-Sensing Receptor Signaling Using Dual Luciferase Assays.- Calcium Imaging in Brain Tissue Slices.- Two-Photon Microscopy to Measure Calcium Signaling in the Living Brain.

- Measuring Calcium-Sensing Receptor Agonist-Driven Insertional Signaling (ADIS) and Trafficking by TIRF Microscopy.- Measuring Calcium Signaling at the Primary Cilia.- Monitoring ER Ca2+ by Luminescence with Low Affinity GFP-Aequorin Protein (GAP).- Analyzing Mitochondrial Calcium Influx in Isolated Mitochondria.- Measuring Calcium Levels in Bone Resorbing Osteoclasts and Bone Forming Osteoblasts.- Quantitative Intravital Calcium Imaging in Mouse Kidney.- Methods for Imaging Intracellular Calcium Signals in the Mouse Mammary Epithelium in 2- and 3-Dimensions.- Live Cell Calcium Imaging in 3D Intestinal Organoids.

- Calcium Imaging and Analysis in Beta Cells in Acute Mouse Pancreas Tissue Slices.- Real-Time Imaging of Calcium Dynamics in Human Sperm after Precise Single-Cell Stimulation.- Calcium Imaging in Drosophila.- Computational Analysis of Calcium Flux Data Using R.