HiBiT-RR and nLucK0 Tagging Systems for Monitoring Targeted Protein Degradation.- Identification of a Ubiquitin-Independent Degron by a Reporter Assay.- Utilization of Functional Degradation Pathways for the Characterization of Ubiquitin-Proteasome System Tool Compounds.- UbiSite Approach: Global Site-Specific Profiling of Canonical and Non-Canonical Ubiquitination in Cells and Tissues.- Chemical and Enzymatic Approaches to C-Terminal Cyclic Imide Formation.- Design and Semisynthesis of Ubiquitin Extension Probes.- Biophysical and Cellular Assays for the Evaluation of Peptidomimetic Inhibitors Based on Degron Sequences.- Multicolor Live-Cell mRNA Imaging Using RNA-Regulated Destabilization Domains.
- Dynamic Control of Cell Signaling with Optogenetic Modulation of Protein Abundance in Living Mammalian Cells.- Reporter Gene Assay for Monitoring BCL6 Reactivation.- Studying Glycan-Dependent ERAD of Misfolded Glycoproteins in Plants.- Protein Depletion in C. elegans Using the Auxin-Inducible Degradation System.- An Auxin-Inducible Degron System for Trypanosomes.- Degrade Green Fluorescent Protein (deGradFP) Method in Trypanosoma brucei.- Production of Purified Proteins Tagged with ssrA-Derived Degrons and Their Degradation by the Escherichia coli ClpXP System.
- Evaluation of Degron Motifs in Escherichia coli Using a Fluorescent Reporter.- Assessment of Bacterial N-Degron Proteolysis by a Dual-Fluorescence Reporter.- SsrA-Based Targeted Protein Degradation in Escherichia coli.