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Degrons : Methods and Protocols
Degrons : Methods and Protocols
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ISBN No.: 9781071655078
Pages: 260
Year: 202608
Format: Trade Cloth (Hard Cover)
Price: $ 293.79
Dispatch delay: Dispatched between 7 to 15 days
Status: Available

HiBiT-RR and nLucK0 Tagging Systems for Monitoring Targeted Protein Degradation.- Identification of a Ubiquitin-Independent Degron by a Reporter Assay.- Utilization of Functional Degradation Pathways for the Characterization of Ubiquitin-Proteasome System Tool Compounds.- UbiSite Approach: Global Site-Specific Profiling of Canonical and Non-Canonical Ubiquitination in Cells and Tissues.- Chemical and Enzymatic Approaches to C-Terminal Cyclic Imide Formation.- Design and Semisynthesis of Ubiquitin Extension Probes.- Biophysical and Cellular Assays for the Evaluation of Peptidomimetic Inhibitors Based on Degron Sequences.- Multicolor Live-Cell mRNA Imaging Using RNA-Regulated Destabilization Domains.


- Dynamic Control of Cell Signaling with Optogenetic Modulation of Protein Abundance in Living Mammalian Cells.- Reporter Gene Assay for Monitoring BCL6 Reactivation.- Studying Glycan-Dependent ERAD of Misfolded Glycoproteins in Plants.- Protein Depletion in C. elegans Using the Auxin-Inducible Degradation System.- An Auxin-Inducible Degron System for Trypanosomes.- Degrade Green Fluorescent Protein (deGradFP) Method in Trypanosoma brucei.- Production of Purified Proteins Tagged with ssrA-Derived Degrons and Their Degradation by the Escherichia coli ClpXP System.


- Evaluation of Degron Motifs in Escherichia coli Using a Fluorescent Reporter.- Assessment of Bacterial N-Degron Proteolysis by a Dual-Fluorescence Reporter.- SsrA-Based Targeted Protein Degradation in Escherichia coli.


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