Why Euglenozoans.- Isolation and Cultivation of Diplonemids.- Preparation of Diplonemid Samples for Microscopy.- Protocol for the Molecular Identification of Autotrophic Euglenids in Environmental Samples.- Culture and Genetic Modification of the Monoxenous Trypanosomatids Crithidia fasciculata and Crithidia bombi.- Poly(A) Profiling for Euglenozoans Using Oxford Nanopore Direct RNA Sequencing, Illustrated by the Example of Euglena gracilis.- Euglenid Extrachromosomal DNA: Assembly and Annotation.- Nuclear Genome Assembly and Annotation of Kinetoplastids.
- Assembly and Annotation of Kinetoplastid and Diplonemid Mitochondrial Genomes.- Cleavage Under Targets and Release Using Nuclease (CUT&RUN) for Trypanosoma brucei Parasites.- Mapping Open Chromatin in Trypanosoma brucei Using ATAC-Seq.- Transcriptomic Analysis of Trypanosomatids.- Small RNA Profiling in Trypanosomes.- Desorption Electrospray Ionization-Mass Spectrometry Imaging (DESI-MSI) of Metabolites in Heart Samples Infected with Trypanosoma cruzi.- Microbiome Profiling in Chagas Disease: Sample Collection, Sequencing, and Analysis.- Metabolomics Analysis of Trypanosomes Using Ion Mobility-Enhanced Mass Spectrometry.
- Exploring Protein Interactomes Using TurboID-Directed Proximity Labeling and Mass Spectrometry.- Mapping Trypanosoma Protein Interactome by Proximity-Dependent Biotinylation.- From Genome to Function: Metabolism-Wide Models of Lifecycle Stages of Parasites.- Enhanced Detection of Homology Using Artificial Intelligence in Euglenids.