1. An ex vivo assay to assess CAR T cell cytotoxicity in viable tumor tissues using a two-step staining protocol Emmanuel intégrative des tumeurs et immunothérapie Donnadieu, Irena Rajnpreht and Lene Vimeux 2. new protocol to measure mitochondria polarization/function at the immunological synapse Manuel Izquierdo, Javier Ruiz Navarro and Victor Calvo 3. Analysis of CAR-T cell-mediated cytotoxicity in solid tumor models Teresa Lozano, Noa Beatriz Martín-Cófreces, Álvaro Gómez-Morón and Celia Martín-Otal 4. Investigation of the in vitro lytic capacity of CAR-T by microscopy' Noa Beatriz Martín-Cófreces, Teresa Lozano, Álvaro Gómez-Morón and Celia Martín-Otal 5. Antigen-specific T cell cytotoxicity toward target tumor cells Chong Sun and Helena Engel 6. In vitro expansion of stem-like memory T cells via D-mannose supplementation Bo Huang, Guideng Li, Yajing Qiu and Cngcong Zhao 7. High-throughput time-lapse imaging of B16 target cell killing by ovalbumin-specific CD8+ T cells.
Hélène Sophie DANIELS-TREFFANDIER, Loic Dupre, Beatriz CHAVES and Nicolas SOCQUET-JUGLARD 8. Evaluation of an intracellular magneto-mechanical approach for regulating dendritic cell activation Yu Cheng, Rui Gao, Wanxin Huang and Yehe Kang 9. Analyzing the effect of NK cells on in-vivo models of tumor dissemination by digital pathology and flow cytometry Massimo Vitale, Paola Orecchia, Guido Ferlazzo, Monica Parodi, Simonetta Astigiano, Valentina Delli Zotti, Filippo Piombo, Stefania Martini, Simona Pigozzi and Paolo Carrega 10. Super-resolution imaging of the NK cell synapse on supported lipid bilayers using MINFLUX nanoscopy. Charles Daniel Murin, Emily Mace, Patrick Ross, Jessica Matthias, Daniel Leaman, Micahel Zwick and Scott Henderson 11. ASSESSMENT OF NK CELL CYTOTOXICITY INDUCED BY IL-15 BASED IMMUNOTHERAPY AGAINST CANCER CELLS Carole Seguin-Devaux, Camille Rolin, Gilles Iserentant and Aubin Pitiot 12. Combined phenotyping and functional analysis of isolated human natural killer cells using spectral flow cytometry Sophie-Christin Linkenbach, Ayline Kübler and Tommaso Torcellan 13. Anti-tumor activity characterization of NK cell subsets by flow cytometry Massimo Vitale, Carola Perrone, Paolo Canevali, Veronica Colamassaro, Elena Giusto, Silvia Tamburini, Gianluca Ubezio, Guido Ferlazzo and Raffaella Meazza 14.
DNA Origami-Decorated Lipid Bilayer Systems for Interrogating the Spatial Requirements underlying B cell Immune-Synapse Formation Bebhinn Treanor, Yuchen Hou, Chung -Yi Tseng, Raysa Orin, Emily Cohen and Leo Chou.