1. Flow cytometry analysis of mitochondrial apoptotic priming with BH3 profiling on senescent cells Joan Montero 2. Methods to study PIDDOsome and caspase 2 signaling Valentina C. Sladky, Andreas Villunger, Felix Eichin and Rotraud Hirschberger 3. Isolation, characterization, and experimental applications of apoptotic bodies in vitro Kristopher Sarosiek, Atomu Yamaguchi, Alejandra Garcia-Carrasco and Xingping Qin 4. Lipid ROS Measurement by Spectral Flow Cytometry based on the ratiometric dye BODIPYTM C11 581/591 Daniel Butcher, Di Yu, Naiqi Wang and Sam Nettelfield 5. CRISPR-Cas9 Screen to Identify Genes Regulating Cell Death Dai Chao Xu and Zi Long Zheng 6. Flow cytometry-assisted discrimination of early apoptotic, late apoptotic and primary necrotic morphological markers in Saccharomyces cerevisiae Didac Carmona-Gutierrez, Tobias Eisenberg, Frank Madeo, Martin N Odabas, Andreas Zimmermann, Katharina Kainz and Maria A Bauer 7.

Cytofluorometric analysis of cell death in primary human neutrophils Thomas Rudel and Andreas Demuth 8. Real-time tracking of cell death in human intestinal organoids: a quantitative imaging approach James Vince 9. SHORT-TERM CULTURE OF HUMAN ENDOSCOPIC SPECIMENS FOR THE STUDY OF CHRONIC INFLAMMATION Sonja Kallendrusch, Werner Julia, Laura Hennig, Florian Lordick, Wirtz Hubert, Frille Armin, Prill Sebastian:, Albrecht Hoffmeister, Hoang Ngoc and Cica Vissiennon 10. In vitro protocols for inducing and assessing endotoxin tolerance in human and murine macrophages Emma M. Creagh, Aoife Smith and Mathew Choaji 11. Assessment of microglial responsiveness after irradiation in mouse organotypic hippocampus or patient-derived tissue cultures Sonja Kallendrusch, Rapp Felicitas, Bahar Djouiai, Steffen Rosahl, Rüdiger Gerlach, Katharina Hess, Franziska Köhler, Ingo Bechmann, Frank Gaunitz, Faramarz Dehghani and Karsten Winter.