Preface ix 1 Temperature for Living Things 1 1.1 Temperature of Individuals 2 1.2 Responses to Temperature Variation at the Cellular Level 11 1.3 Significance of Intracellular Thermometry 13 References 14 2 Fluorescent Molecular Thermometers 17 2.1 The Basics of Fluorescence 18 2.2 Responses of Fluorescent Molecular Thermometers 19 2.3 Small Organic Molecules Involving Intersystem Crossing 21 2.4 Small Organic Molecules with a Rotating Substituent Group 25 2.
5 Reactive Small Organic Molecules 36 2.6 Viscosity-Sensitive Small Organic Molecules 38 2.7 Other Small Organic Molecules 39 2.8 Organometallic Complexes 41 2.9 Excimers and Exciplexes 49 2.10 Host-guest Interactions 54 2.11 Synthetic Polymers 57 2.12 Nucleic Acids (DNA and RNA) 76 2.
13 Peptides 79 2.14 Fluorescent Proteins 79 2.15 Non-covalent Systems Based on Thermo-responsive Self-assembly and an Environment-sensitive Fluorophore 81 2.16 Inorganic Nanomaterials 84 2.17 Hybrid Nanomaterials 95 References 97 3 Intracellular Thermometry with Fluorescent Molecular Thermometers 109 3.1 Early Attempts for Cellular Thermometry 109 3.2 Introduction of Fluorescent Molecular Thermometers into a Living Cell 112 3.3 Cytotoxicity Assessment 121 3.
4 Practice of Intracellular Thermometry with Fluorescent Molecular Thermometers 126 3.5 Intracellular Thermometry with Organelle-targeted Fluorescent Molecular Thermometers 146 3.6 Intracellular Thermometry of Brown Adipocytes 156 3.7 Application of Intracellular Thermometry in Various Biological Fields 163 References 175 4 Cellular Thermometry Based on Non-fluorometric Principles 183 4.1 Infrared Thermometry 185 4.2 Photoacoustic Thermometry 186 4.3 Raman Thermometry 188 4.4 Use of Transmission Spectroscopy 191 4.
5 Thermocouple 193 4.6 Resonant Thermal Sensor 197 4.7 Bimaterial Microcantilever 199 4.8 Thermistor 200 References 202 5 Reliability Issue in Intracellular Thermometry 205 5.1 Sensitivity and Temperature Resolution 205 5.2 Functional Independency of Fluorescent Molecular Thermometers 208 5.3 Preparation of a Calibration Curve of Fluorescent Molecular Thermometers 212 5.4 Objection to Endogenous Thermogenesis 215 5.
5 Possible Artifacts in Near-infrared Luminescent Thermometry and Proposal for Reliable Thermometry 233 References 236 6 Applications of Intracellular Thermometry 241 6.1 Creation of New Biological Concepts 241 6.2 In vivo Temperature Measurements 242 6.3 Thermal Medicine at a Single-cell Level 250 6.4 Utilization of Nanoheater/Fluorescent Thermometer Hybrids in Biotechnology 258 6.5 Accurate Measurements of Absolute Intracellular Temperature 262 6.6 Simultaneous Monitoring of Intracellular Temperature and the Concentration of a Chemical Species Related to a Temperature Variation Inside a Living Cell 263 6.7 Intracellular Thermometry of Plant Cells 266 References 268 Appendix 1 Review and Feature Articles on Fluorescent Molecular Thermometers and Intracellular Thermometry in General 273 Appendix 2 Comprehensive Collection of Fluorescent Polymeric Thermometers Based on the Combination of a Thermo responsive Polymer and an Environment-sensitive Fluorophore 275 Appendix 3 Comprehensive Collection of Fluorescent Nanogel Thermometers Based on the Combination of a Thermo-responsive Polymer and an Environment-sensitive Fluorophore 295 Appendix 4 Comprehensive Collection of Non-covalent Fluorescent Temperature Sensing Systems Based on the Combination of a Thermo-responsive Polymer and an Environment-sensitive Fluorescent Compound 301 Appendix 5 Comprehensive Collection of Fluorescent Polymeric Logic Gates Based on the Combination of a Thermo-responsive Polymer and an Environment-sensitive Fluorophore 307 Appendix 6 Solid Inorganic Nanostructures Showing Temperature dependent Emission Properties 321 Appendix 7 Tips for Intracellular Thermometry with Fluorescent Polymeric Thermometers Developed in the Author''s Laboratory 323 Index 325.