1. Generation and functional evaluation of STAb-T cells Luis Alvarez-Vallina 2. A syngeneic orthotopic mouse model of metastatic colorectal cancer Christel Devaud 3. High-parameter T-cell spectral phospho flow cytometry Joshua Brody and Gvantsa Pantsulaia 4. Measuring T-cell/CAR T-cell bystander tumor killing in vitro and in vivo Joshua Brody, Matthew Lin and Daniel Charytonowicz 5. Quantification and functional assessment of antigen(-) cancer cell variants Joshua Brody, Gvantsa Pantsulaia, Ivan Odak and Xie Xinping 6. T-cell and CAR T-cell Cas9-RNP/nucleofection-mediated gene editing Joshua Brody, Gvantsa Pantsulaia and Moah Sohn 7. Peripheral blood mononuclear cell (PBMC)- based functional evaluation of human T cell response to suppressive cells and immune-oncology therapeutics Maria G.
Castro, Kaushik Banerjee, Pedro R. Lowenstein, Mahmoud Alghamri, Brandon McClellan and Jorge Peña Agudelo 8. Integrative analysis of lung multiparametric flow cytometry data to study immune cell dynamics in tumors Romina Goldszmid and Romina Araya 9. Multiparametric staining: Combined application of immunofluorescence and in-situ hybridization Barbara Seliger, Marcus Bauer, Andreas Wilfer, Clara Zöllig and Claudia Wickenhauser 10. Extracellular matrix and morphology assessment method on live 3D spheroids of thyroid carcinoma Barbara Seliger, Mario Udinotti, Udo Sieboldts and Christoforos Vaxevanis 11. Hema-CRISPR: A clone-based protocol for effective genetic editing of hematopoietic stem cells Barbara Seliger, Christoforos Vaxevanis, Mario Udinotti, Dimitris Kokoretsis, Andreas Wilfer, Marcus Bauer and Claudia Wickenhauser 12. Patient-derived models of tumor-immune cell interactions Zlatko Trajanoski 13. MDSC isolation from human tumors Viktor Umansky, Feyza Gul Ozbay Kurt and Jochen Utikal 14.
Extracellular vesicles isolation from human maternal fluids Sheila Spada 15. Finding a Needle in a Haystack: Measures of Circulating Tumor DNA (ctDNA) to Guide Treatment Decisions in Oncology Lotze, Michael T, Genia Dubrovsky, Jeff Szymanski, Aadel A. Chaudhuri, Jose Zevallos, Noah Earland, Peter Harris and Pradeep Chauhan.