Unlocking the secrets of extracellular vesicles and exosomes: A journey into the cutting-edge frontier of cellular communication provides a comprehensive overview of the latest advances and research on extracellular vesicles (EVs) and exosomes. The book begins with a historical introduction to the field and how this has evolved since EVs were first observed, before exploring their cellular mechanisms and approaches to study their isolation, characterization, and function. It then delves into exosomes in health and disease, considering their role across cancer, regenerative medicine, neurological disorders, and metabolic disorders, highlighting their potential as biomarkers for disease diagnosis. The book moves on to discuss cutting-edge technology for the engineering of EVs and exosomes, considering how the integration of AI, machine learning, and nanotechnology can be utilized to expand the therapeutic capabilities of EV and exosome-based applications. It then closes with chapters covering novel applications in nutraceutical and cosmetic production, and a look towards the future potential of EVs and exosomes. Unlocking the secrets of extracellular vesicles and exosomes offer a thorough exploration of EV and exosome research, covering various dimensions from basic science to clinical applications and future perspectives. It is an ideal guide for researchers working across a diverse range of disciplines, including cell biology, molecular biology, biotechnology, therapeutics, and biomedicine.