1. In situ imaging of pulmonary invariant Natural Killer T cells in precision cut lung slices Claudia Burrello, Karin E. de Visser and Tom Langelaar 2. Immunofluorescent microscopy to quantify and characterize chromosomal instability Pippa F. Cosper, Kathryn Jones, Yeseo Choi, Radchanon Leelasukseree, Nikki Messick, Ava Bryan and Sophie Bice 3. Spatial techniques for understanding the tumour microenvironment Florent Ginhoux, Margaux GARDET and Kevin Mulder 4. Very high-flex immunophenotyping using co-linear cytofluorometric evaluation Benjamin Izar 5. Large-scale CRISPR-Cas9 screens to define regulators of immune checkpoint biology Benjamin Izar 6.
Assessment of cytoplasmic dsDNA for cellular immunogenicty Rongze Olivia Lu, Raymond Sun, Oishika Das and Isha Mondol 7. Harmonization of a multiparametric flow cytometry panel for immunological monitoring on PBMC and whole blood samples: a real-life example Iole Macchia Sr., Francesca Urbani and Valentina La Sorsa 8. Quantitative assessment of arterial remodeling from confocal microscopy image analysis of the mid-gestation mouse decidua Alexander J. Muller and Dema Alghabban 9. Phenotypic characterization and function of tumor infiltrating and peripheral B cells in cancer patients Paola Nistico, Maria Rosado, Giuseppe Frisullo, Giulia Campo and Miriam Petrella 10. Methodological Advances to analyze Cancer-Associated Fibroblast-derived ECM effects on Macrophage Polarization Paola Nistico, Annalisa Tocci, Nicla Porciello, Belinda Palermo, Lorenzo Valenti, Anna Di Carlo and Francesca Di Modugno 11. Lymphocyte migration/invasion assays Louis M.
Weiner, Rachael Maynard and Alex Lekan 12. Flow-cytometry-based assay to study CD8+ T cell cytotoxicity against tumor cells. Roberta Zappasodi, Alexey V Sarapulov and Marc-Anthony Rodriguez 13. High dimensional flow cytometry for conventional and unconventional T cell populations Maria Paula Roberti, Andrea Leufgen, Conrad Rauber, Annika Steitz, Paula Daza and Maria Paz Gavilan 14. Optimized Phospho-Flow Cytometry for Quantitative Analysis of Early TCR-Proximal Signaling: Implications for T Cell Function in Onco-Immunology Paola Nistico, Nicla Porciello, Annalisa Tocci, Giulia Campo and Belinda Palermo.