Intestinal stem cells: regulation, plasticity, and therapeutic Potential.- In silico single-cell framework for modelling intestinal stem and transit-amplifying progenitor cells dynamics.- Using in silico pseudobulk differential expression and trajectory analysis of single cell RNA sequencing to reveal stem cell state transitions in colonic inflammation.- Cross-species translation of drug-Induced toxicity in the small intestine using mathematical modelling.- A computational framework to resolve stem cell gene dynamics during intestinal inflammation.- Animal-free intestinal epithelial 2D "Organoid-Sheet" cultures using invasion.- Primary cultures of foetal epithelial progenitors.- An integrated workflow for culturing and AI-based profiling of mouse intestinal epithelium.
- Updated protocols for 3D and 2D culture and histological analysis of intestinal organoids.- Rapid and efficient genetic manipulation of intestinal organoids under transient two-dimensional culture conditions.- Human small intestinal organoids differentiation protocols towards all mature epithelial lineages.- Mass spectrometry - based proteomics of intestinal organoids.- Organoid culture model for the study of adult slow - cycling putative intestinal stem cells.- Intestinal organoids culture in synthetic hydrogels.- Live Fluorescence Lifetime Imaging Microscopy (FLIM) of the epithelial polarity and cell-specific segmentation in the small intestinal organoids.- Whole-mount immunostaining of human intestinal organoids.
- Monitoring calcium dynamics in pig intestinal organoids with FLIM and FRET.- Methods for processing stem cell-derived organoids for histological and immunofluorescence staining.- Stemness in colon cancer.- Deep insights without clearing: an optimized protocol for patient-derived whole-organoid immunofluorescence imaging.- Comprehensive isolation strategy for intestinal epithelial and fibroblast cells from both healthy and tumour human tissues.- A tissue explant model for colon adenocarcinoma.- A straightforward and reliable mouse model of colorectal cancer by orthotopic transplantation.