Cas9 Ribonucleoproteins (RNPs) for Gene Deletion.- Generation of Seamless Point Mutations with Cas9 RNP and pTel-hygR Plasmid in Aspergillus fumigatus.- Marker-Free CRISPR/Cas9 RNP Transformation for Gene Disruption in Aspergillus fumigatus.- CRISPR-Cas9 and Microhomology-Mediated End-Joining for Biosynthetic Pathway Reconstruction in Aspergillus fumigatus.- PCR Typing and Genome Sequencing of Aspergillus fumigatus.- Isolation of Nucleic Acids from Aspergillus fumigatus for Sequencing.- Methods for Large-Scale Intraspecific Analyses of Single Nucleotide Polymorphisms in the Mitochondrial Genomes of Aspergillus fumigatus.- Development and Validation of a Real-Time PCR Assay for the Detection of Aspergillus fumigatus on Panther Fusion System.
- RAPD-PCR for Typing and Identification of Aspergillus fumigatus.- Electrophoretic Mobility Shift Assay in Aspergillus fumigatus.- Chromatin Immunoprecipitation in Aspergillus fumigatus.- Micropatterning Substrates Using Deep UV Lithography: A Model to Study Host-Pathogen Interactions of A. fumigatus with Adherent Cells.- Bronchoalveolar Lavage Fluid Collection in Invasive Pulmonary Aspergillosis Murine Model for Downstream Proteomic Analysis.- Galleria mellonella to Study Aspergillus Infections and Antifungal Efficacy by Traditional Readouts and Novel BL-Imaging Techniques.- Molecular Biosensing of the Aspergillus fumigatus Cell Wall Integrity Pathway Using the (p)agsA::luc (Luciferase) Reporter System.
- Evaluation of Aspergillus fumigatus Metabolic Activity In Vitro: A Comparison Between Resazurin and XTT Methods.- Aspergillus fumigatus Cell Wall: Current Advances in Structural Elucidation Methods.- Isolation and Purification of Extracellular Vesicles from Aspergillus fumigatus.